Vitrification

Vitrification is a technology used for freezing embryos and eggs. The embryologist can use the frozen egg or embryo for later use. The use of vitrification technology is not limited to fertility space. It can also convert a crystalline structure into a smooth one

While freezing any cell in a lab, the prime focus is to avoid ice crystal formation due to subzero temperatures. The sharp edges of ice crystals can damage the cell membrane. Also, water inside the cells expands in volume when it becomes ice, causing rupture of the cell ice crystals and posing significant and fatal problems for cells.
Vitrification is one of the most effective solutions to this problem.

Vitrification is a three-step technology

Step 1

The technician exposes eggs or embryos to high concentrations of cryoprotectants. These cryoprotectants allow rapid dehydration of cells.

Step 2

It involves the loading of the eggs or embryos into tiny storage devices to facilitate ultra-rapid cooling

Step 3

These storage devices cool the eggs and embryos rapidly. The high cooling rate allows the embryos/eggs and the surrounding fluid to turn to a glass-like substance. This glass-like substance can protect the embryo/eggs from damage and give survival rates above 90%.

De freezing

When people need their vitrified embryos or eggs, they are brought back to 37°C and then allowed for rehydration. This procedure takes just 20 minutes. The technician puts the warmed cells in the incubator. The embryologist can transfer the warmed embryos immediately; inject the warmed eggs with a single sperm within 3-4 hours.